This lab manual emphasizes the basic principles of diagnostic microbiology for students preparing to enter the allied health field. Students are led through a series of exercises that allow them to learn basic microbiology techniques and practice safety in the laboratory and hospital environment. It is primarily oriented toward meeting the interests and needs of those who will be directly involved in patient care and wish to learn how microbiological principles should be applied in the practice of their professions.
The authors articulate the purposes and function of the clinical microbiology laboratory in the diagnosis of infectious diseases. Exercises illustrate the nature of laboratory procedures used for isolation and identification of infectious agents as well as the principles of asepsis, disinfection and sterilization. The role of the health professional is highlighted in regard to appropriate collection of clinical specimens and application of aseptic and disinfectant techniques as they relate to patient care.
Contents: فهرست فصول
CoverTitle PageCopyrightContentsPrefaceOrientation to the Microbiology LaboratoryPart One Basic Techniques of MicrobiologyExercise 1. The MicroscopeExercise 2. Handling and Examining CulturesExercise 3. Simple Stains and Wet-Mount PreparationsExercise 4. Gram StainExercise 5. Acid-Fast StainExercise 6. Culture MediaExercise 7. Streaking Technique to Obtain Pure CulturesExercise 8. Subculture TechniquesPart Two Destruction of MicroorganismsExercise 9. Moist and Dry HeatExercise 10. The AutoclaveExercise 11. DisinfectantsExercise 12. Antimicrobial Agent Susceptibility Testing and ResistancePart Three Diagnostic Microbiology in ActionExercise 13. Primary Media for Isolation of MicroorganismsExercise 14. Some Metabolic Activities of BacteriaExercise 15. Activities of Bacterial EnzymesExercise 16. Antigen Immunoassays for Detection and Identification of MicroorganismsExercise 17. Principles of Nucleic Acid Assays and Multiplex Syndrome Panel Testing for the Diagnosis of Infectious DiseaseExercise 18. Principles and Applications of MALDI-TOF Mass Spectrometry for the Rapid Identification of Bacteria and FungiExercise 19. StaphylococciExercise 20. Streptococci, Pneumococci, and EnterococciExercise 21. Haemophilus, Corynebacteria, and BordetellaExercise 22. Clinical Specimens from the Respiratory TractExercise 23. The Enterobacteriaceae (Enteric Bacilli) and Other Clinically Important Gram-Negative BacilliExercise 24. Clinical Specimens from the Intestinal TractExercise 25. Urine Culture TechniquesExercise 26. Neisseria and SpirochetesExercise 27. Clinical Specimens from BloodPart Four Microbial Pathogens Requiring Special Laboratory Techniques; Serodiagnosis of Infectious Disease; Principles and Practices of Infection PreventionExercise 28. Anaerobic BacteriaExercise 29. MycobacteriaExercise 30. Mycoplasmas, Rickettsiae, Chlamydiae, Viruses, and PrionsExercise 31. Fungi: Yeasts and MoldsExercise 32. Protozoa and Animal ParasitesExercise 33. Serodiagnosis of Infectious DiseaseExercise 34. Principles and Practices of Infection PreventionSelected Literature and SourcesIndex